The impacts of molecular motor traffic jams.

M uch of a modern person’s day is spent trying to get from point A to point B. So, too, in the cell, much time and energy is expended on shuttling organelles, protein complexes, and mRNA, called “cargos,” from point A to point B. We know that vehicle traffic slows down when roads get jammed with high-volume congestion. The traffic analogy begs the question: Do cellular highways get jammed? This is the question being probed by Leduc et al. in PNAS (1). Efficient cellular transport is made along “cellular highways” in the form of a cytoskeletal network of proteinacous filaments called microtubules (2, 3). The microtubules crisscross the cell in a radial array to connect distal regions. The cell has “motor proteins” to traverse the highways and carry cargos. Motor proteins are nanoscale machines made from two polypeptide chains folded and wound together (Fig. 1A). These protein dimers have enzymatic feet (called “motor heads”) that bind to the microtubule filament and use ATP to cause alternative stepping in a hand-over-hand style (Fig. 1A) (4, 5). Numerous biophysical studies have discovered a number of amazing properties about motor proteins, including their step size, their maximum force, their velocity, and the “run length” for how far single motors can run (4, 6–9). Together, the motor proteins and microtubules ensure the temporal and spatial demands for on-time delivery of cargos in the cell. The question of motor protein traffic jams is an important one because cells depend on the cargo transport provided by the microtubule–motor network inside cells. When intracellular transport breaks down, the results are often neuromuscular diseases, such as amyotrophic lateral sclerosis (ALS, also known as Lou Gehrig’s Disease) (10). This is because motor neurons that connect the cell body in the spinal cord to the muscles of the body can be up to a meter in length. Without proper intracellular transport, goods and supplies cannot get from the cell body, where they are produced, to the tip of the axon, where they are needed to maintain the neural–muscular junction connecting nerve to muscle. Without replenishment of supplies, the tip of the axon begins to retract, and the muscle can no longer be controlled, resulting is paralysis. The most identifiable modern case of ALS is distinguished physicist Dr. Stephen Hawking. The report by Leduc et al. in PNAS demonstrates that motor protein traffic jams can exist, given the right conditions (1). For this demonstration, the authors use Kip3, a yeast kinesin-8 family motor. Kip3 not only walks but is also known to destroy microtubules from their ends (depolymerization) in cells (11). The Kip3 used in this assay was not able to destroy the microtubules because the filaments were doubly stabilized, so the motors only walked along microtubules. Kip3 kinesins were picked for this study for a number of properties that were likely to result in traffic jams. (i) They are highly processive, meaning they take many steps (more than 2,000 of 8 nm each) before they fall off (dissociate from) the filament (Fig. 1B). This means they get stuck on the highway for a long time; there are few “exit ramps” (Fig. 1C). (ii) When Kip3 kinesins come to the end of a microtubule track, they pause before they dissociate from the end (Fig. 1B). The analogous situation for a motor vehicle traffic jam is that they come to a barrier, such as construction or an accident, that blocks several lanes and only allows a few cars to pass (Fig. 1C). (iii) Kip3’s motility and binding properties can be tuned with salt concentration. At higher salt, they dissociate from the microtubule faster both from the middle of the track and at the end, giving them more exit ramps along the filament and a smaller “construction zone” at the end of the filament. With these tunable attributes, Leduc et al. demonstrate and measure the evolution of a nanoscale traffic jam (1). Kip3 traffic jams along the microtubule built from the end (construction zone) and caused a pileup that grows in size (Fig. 1). Before hitting the jam, kinesin motors moved at top speed, but once hitting the jam the motors slowed down, just as cars do in a vehicular traffic jam (Fig. 1). In addition to the slow-down, motor proteins also dissociated from the ON-RATE OFF-RATE